The SPARCL™ Assay

The SPARCLassay is a rapid homogenous immunoassay which, unlike ELISA, requires no protracted incubation and washing steps.

SPARCL™ assays use two biomarker-specific antibodies. One is conjugated to horseradish peroxidase (HRP); the other to acridan, a chemiluminescent substrate. When HRP and acridan conjugates bind to the target biomarker they are brought into proximity. With the addition of hydrogen peroxide, HRP catalyzes oxidation of proximal acridan causing a flash of luminescence that is proportional to biomarker concentration.

Advantages of SPARCL™ compared to ELISA are:

  • Single incubation step
  • No wash steps.
  • Single point calibration
  • Curve fitting software is not required.

 

SPARCL™ REVIEWS

Johnson & Johnson Case Study for SPARCL™ (pdf)

Monsanto Company SPARCL™ Case Study (pdf)

Current Assays

SPARCL™ tests are available for use with the VetBio-1 single tube/double injector luminometer.

Current Sirius-L assays are available for:

  • Canine CRP
  • Canine Haptoglobin
  • Canine S100-A8
  • Canine Lactoferrin
  • Canine Procalcitonin
  • Canine Thymidine Kinase 1
  • Canine Troponin 1
  • Feline SAA
  • Equine SAA

We have many more assays and are keen to collaborate with laboratories to develop novel specific biomarker tests.

Instrumentation

VetBio 1

The VetBio 1 is now available.  It has bespoke software capable of automating the measurement of a wide range of veterinary biomarkers using the SPARCLTM method using onboard reference standard curves.

Measurement of each sample takes only two seconds and is initiated by simply closing the sample draw. Results are then displayed in the relevant units and can be stored on the instrument or transferred to inhouse software via a memory stick.

Conscious of space constraints in many laboratories, the VetBio 1 is completely self-contained and has a small footprint measuring only 35×15 cm.

Lu-mini

The Lu-mini has been designed for POC use in veterinary practices. It has been developed to work with the same SPARCL™ assay currently available for the VetBio 1. Like the larger reference laboratory instrument, the Lu-mini is self-contained with customised software onboard. It has a smaller footprint than the VetBio 1, measuring only 23x16cm.

Resulting from feedback given by first opinion veterinary practices, we are currently working on an alternative reagent delivery system for the Lu-mini to work with only fixed volume pipettes, thereby making the test much better suited to clinic use.

SPARCLTM assays are protected around the world by the following patents.

US Patent Nos.  : 6,696,569; 7,682,839; 8,106,230, 7,732,153; 7,923,213; 8,012,705; 8,124,362; 7,799,534; 7,923,214; 8,076,092

Australian Patent No. :  2004200649. 

Canadian Patent No. :  2,300,071 

Chinese Patent No.  :  ZL 200780022839.3

European Patent No.  :  EP 1015461B1.

Japanese Patent No.  :  4,431,272